Difference between revisions of "Part:BBa K4060109"
 
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BBa_J23106 + BBa_K4060106
 
BBa_J23106 + BBa_K4060106
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The gene fragment Pre C2 is composed of BBa_J23106 and the Pre B3. We digested the vector fragment Pre B3 with EcoRI and XbaI and the insert fragment BBa_J23106  with EcoRI and SpeI. We ligated these fragments together to form Pre C2.
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<img src="https://2021.igem.org/wiki/images/3/30/T--NYCU-Taipei--PreC2.png" height="475px" width="750px"/>
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Agarose electrophoresis in order to verify Pre C2.
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Lane 1, 100bp ladder. Lane 2,3, Pre C2 digested with NheI.
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<!-- Add more about the biology of this part here

Latest revision as of 14:44, 7 December 2021


Pre C2

BBa_J23106 + BBa_K4060106

The gene fragment Pre C2 is composed of BBa_J23106 and the Pre B3. We digested the vector fragment Pre B3 with EcoRI and XbaI and the insert fragment BBa_J23106 with EcoRI and SpeI. We ligated these fragments together to form Pre C2.

Agarose electrophoresis in order to verify Pre C2. Lane 1, 100bp ladder. Lane 2,3, Pre C2 digested with NheI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 754
    Illegal AgeI site found at 866
  • 1000
    COMPATIBLE WITH RFC[1000]