Difference between revisions of "Part:BBa K4060110"
 
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Since bioparts may behave differently in different host strains, any team who wish to integrate this kill switch system into their designs can use Construct 2 (BBa_K4060111) to roughly estimate MazE expression level in various condition.
 
Since bioparts may behave differently in different host strains, any team who wish to integrate this kill switch system into their designs can use Construct 2 (BBa_K4060111) to roughly estimate MazE expression level in various condition.
 
After that, a constitutive promoter with appropriate strength could be selected to construct a MazF constant expression system. Strength of constitutive promoters can be test out with an RFP reporter system, and we found that BBa_J23100 constitutive promoter family on BBa_J61002 reporter plasmid is useful for this purpose. A promoter that shows a slightly higher level of RFP expression in comparison with that of Construct 2 (BBa_K4060111) under L-arabinose induction (1mM) or low temperature (20℃) should be chosen. Integrate Construct 1 (BBa_K4060110) with the aforementioned MazF constant expression system in the same plasmid, and the kill switch system is completed.
 
After that, a constitutive promoter with appropriate strength could be selected to construct a MazF constant expression system. Strength of constitutive promoters can be test out with an RFP reporter system, and we found that BBa_J23100 constitutive promoter family on BBa_J61002 reporter plasmid is useful for this purpose. A promoter that shows a slightly higher level of RFP expression in comparison with that of Construct 2 (BBa_K4060111) under L-arabinose induction (1mM) or low temperature (20℃) should be chosen. Integrate Construct 1 (BBa_K4060110) with the aforementioned MazF constant expression system in the same plasmid, and the kill switch system is completed.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:30, 7 December 2021


Const1

Since bioparts may behave differently in different host strains, any team who wish to integrate this kill switch system into their designs can use Construct 2 (BBa_K4060111) to roughly estimate MazE expression level in various condition. After that, a constitutive promoter with appropriate strength could be selected to construct a MazF constant expression system. Strength of constitutive promoters can be test out with an RFP reporter system, and we found that BBa_J23100 constitutive promoter family on BBa_J61002 reporter plasmid is useful for this purpose. A promoter that shows a slightly higher level of RFP expression in comparison with that of Construct 2 (BBa_K4060111) under L-arabinose induction (1mM) or low temperature (20℃) should be chosen. Integrate Construct 1 (BBa_K4060110) with the aforementioned MazF constant expression system in the same plasmid, and the kill switch system is completed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1112
    Illegal XbaI site found at 1128
    Illegal SpeI site found at 1382
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1112
    Illegal NheI site found at 125
    Illegal SpeI site found at 1382
    Illegal NotI site found at 1119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1112
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1179
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1112
    Illegal XbaI site found at 1128
    Illegal SpeI site found at 1382
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1112
    Illegal XbaI site found at 1128
    Illegal SpeI site found at 1382
  • 1000
    COMPATIBLE WITH RFC[1000]