Difference between revisions of "Part:BBa K4060111"
 
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To test how our kill switch design works, the expression of MazE and TetR can be evaluated by testing the RFP intensity under different L-arabinose concentrations and temperatures from Construct 2 & 3. We initially expected the bacteria with Construct 2 would show fluorescent red in LB medium (without L-arabinose). However, there was no red color in the colony of the bacteria, which means the RFP gene didn’t express. After using a photometer, the value of RFP intensity (RFP/OD600) is almost zero according to Fig.4, which is out of our expectation.
 
To test how our kill switch design works, the expression of MazE and TetR can be evaluated by testing the RFP intensity under different L-arabinose concentrations and temperatures from Construct 2 & 3. We initially expected the bacteria with Construct 2 would show fluorescent red in LB medium (without L-arabinose). However, there was no red color in the colony of the bacteria, which means the RFP gene didn’t express. After using a photometer, the value of RFP intensity (RFP/OD600) is almost zero according to Fig.4, which is out of our expectation.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:32, 7 December 2021


Const2

To test how our kill switch design works, the expression of MazE and TetR can be evaluated by testing the RFP intensity under different L-arabinose concentrations and temperatures from Construct 2 & 3. We initially expected the bacteria with Construct 2 would show fluorescent red in LB medium (without L-arabinose). However, there was no red color in the colony of the bacteria, which means the RFP gene didn’t express. After using a photometer, the value of RFP intensity (RFP/OD600) is almost zero according to Fig.4, which is out of our expectation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1661
    Illegal AgeI site found at 1773
  • 1000
    COMPATIBLE WITH RFC[1000]