Difference between revisions of "Part:BBa K3724014"

Latest revision as of 03:09, 22 October 2021

SO_3468+ribBA+ribE cluster

This gene cluster consists of the genes SO_3468, rib ba and ribe which are genes that encode proteins involved in the riboflavin biosynthesis pathway in Shewanella oneidensis MR-1. This gene cluster makes up a component of the riboflavin synthesis gene cluster (BBa_K3724015).

Usage and Biology

Shewanella oneidensis MR-1 are gram-negative bacteria at the center of studies of microbial reduction due to their ability to transfer electrons extracellularly to reduce materials such as graphene oxide (GO) [1]. Such characteristics have made S. oneidensis MR-1 an organism of interest in microbial fuel cells for bioelectricity generation and potential applications in bioremediation [2]. Two extracellular electron transfer pathways have been identified in the reduction of GO by Shewanella oneidensis MR-1. These are indirect electron transfer, mediated by secreted electron shuttles, and direct extracellular electron transfer (DET) which involves direct contact with the extracellular material [3].

The SO_3468+ribBA+ribE cluster encodes the riboflavin synthase alpha subunit (SO_3468) which catalyzes the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil from two molecules of 6,7-dimethyl-8-ribityllumazine, the GTP cyclohydrolase-2 (ribA) which catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate , formate and pyrophosphate, 3,4-dihydroxy-2-butanone-4-phosphate synthase (ribB) which catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate and riboflavin synthase beta subunit (RibE) which catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2- butanone 4-phosphate. These proteins are essential for the production of riboflavin in the riboflavin biosynthesis pathway of S. oneidensis . It has been proposed that flavins may act as electron shuttles in the reduction of extracellular material by S. oneidensis MR-1 [4]. Therefore, this gene cluster was used in the construction of the riboflavin synthesis gene cluster (BBa_K3724015) to increase the production of riboflavin in S. oneidensis MR-1 for increased rate of reduction of graphene oxide.

Sequence and Features

References

[1] Wang, G.; Qian, F.; Saltikov, C. W.; Jiao, Y.; Li, Y. Microbial Reduction of Graphene Oxide by Shewanella. Nano Research 2011, 4, 563–570.
[2] Schwalb, C.; Chapman, S. K.; Reid, G. A. The Tetraheme Cytochrome Cyma Is Required for Anaerobic Respiration with Dimethyl Sulfoxide and Nitrite in Shewanella Oneidensis. Biochemistry 2003, 42, 9491–9497.
[3] Lin, T.; Ding, W.; Sun, L.; Wang, L.; Liu, C.-G.; Song, H. Engineered Shewanella Oneidensis-Reduced Graphene Oxide Biohybrid with Enhanced Biosynthesis and Transport of Flavins Enabled a Highest Bioelectricity Output in Microbial Fuel Cells.
[4] Kouzuma, A.; Kasai, T.; Hirose, A.; Watanabe, K. Catabolic and Regulatory Systems in Shewanella Oneidensis MR-1 Involved in Electricity Generation in Microbial Fuel Cells. Frontiers in Microbiology 2015, 6.