Difference between revisions of "Part:BBa K3898166:Design"
 
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This sequence was optimized for <i>E. coli BL21</i> using the Codon Optimization Tool from the genscript website.
 
This sequence was optimized for <i>E. coli BL21</i> using the Codon Optimization Tool from the genscript website.
−
 
  
 
===Source===
 
===Source===

Latest revision as of 02:35, 22 October 2021


A delicate modular enzymes system to enhance PET plastic degradation at mild temperature


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal XbaI site found at 1232
    Illegal XbaI site found at 3518
    Illegal PstI site found at 2153
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2153
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2493
    Illegal BamHI site found at 4180
    Illegal XhoI site found at 875
    Illegal XhoI site found at 3143
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal XbaI site found at 1232
    Illegal XbaI site found at 3518
    Illegal PstI site found at 2153
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal XbaI site found at 1232
    Illegal XbaI site found at 3518
    Illegal PstI site found at 2153
    Illegal NgoMIV site found at 360
    Illegal NgoMIV site found at 1378
    Illegal AgeI site found at 74
    Illegal AgeI site found at 2191
    Illegal AgeI site found at 3545
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was optimized for E. coli BL21 using the Codon Optimization Tool from the genscript website.

Source

BBa_K3898152, BBa_K3898163, BBa_K3898165

References