Difference between revisions of "Part:BBa K3771076"
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<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:35%;"> | <img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:35%;"> | ||
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− | <p align="center">Fig. 1 L-cysteine sulfonic acid synthase catalyzes the reaction that turns O-phospho-L-serine into L-cysteine sulfonic acid. CDO1, L-cysteine dioxygenase; CSAD, L-cysteine sulfinic acid decarboxylase</p> | + | <p align="center">Fig. 1. L-cysteine sulfonic acid synthase catalyzes the reaction that turns O-phospho-L-serine into L-cysteine sulfonic acid. CDO1, L-cysteine dioxygenase; CSAD, L-cysteine sulfinic acid decarboxylase</p> |
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− | <br>pSAA-<i>P<sub> | + | <br>pSAA-<i>P<sub>lacI</sub>-csad</i><br> |
<div style="width=100%; display:flex; align-items: center; justify-content: center;"> | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
<img src="https://2021.igem.org/wiki/images/8/86/T--NCKU_Tainan--CSAD-PCR.png" style="width:35%;"> | <img src="https://2021.igem.org/wiki/images/8/86/T--NCKU_Tainan--CSAD-PCR.png" style="width:35%;"> | ||
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− | <p align="center">Fig. 2 Confirmation of <i>csad</i> fragment by PCR. M: Marker; Lane 1: <i>csad</i> (1368 bp)</p> | + | <p align="center">Fig. 2. Confirmation of <i>csad</i> fragment by PCR. M: Marker; Lane 1: <i>csad</i> (1368 bp)</p> |
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<img src="https://2021.igem.org/wiki/images/4/48/T--NCKU_Tainan--CSAD1-Vactor-digestion.png" style="width:35%;"> | <img src="https://2021.igem.org/wiki/images/4/48/T--NCKU_Tainan--CSAD1-Vactor-digestion.png" style="width:35%;"> | ||
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− | <p align="center">Fig. 3 Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)</p> | + | <p align="center">Fig. 3. Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)</p> |
Latest revision as of 03:17, 22 October 2021
PlacI-CSAD
Description
PlacI-csad is a composite part consisting of the lacI promoter and the csad sequences. This part was used in in vivo testing of taurine production. The sequence for csad enzyme and lacI promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).
Biology
lacI promoter constitutively facilitates the expression of CSAD enzyme.
Fig. 1. L-cysteine sulfonic acid synthase catalyzes the reaction that turns O-phospho-L-serine into L-cysteine sulfonic acid. CDO1, L-cysteine dioxygenase; CSAD, L-cysteine sulfinic acid decarboxylase
Usage
Characterization
pSAA-PlacI-csad
Fig. 2. Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)
Fig. 3. Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 476
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 261