Difference between revisions of "Part:BBa K3771013"
 
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<img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;">
 
<img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;">
 
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   <p align="center">Fig. 1. Double digestion of <i>P<sub>lacI</sub></i>-OFP</p>
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   <p align="center">Fig. 1. Double digestion of <i>P<sub>lacI</sub>-ofp</i></p>
  
  

Latest revision as of 01:33, 22 October 2021


PlacI-OFP


Description

PlacI-ofp is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.

Biology

Without the presence of the LacI repressor, the lacI promoter facilitates the constitutive expression of OFP.

Characterization

Double digestion results are shown in Fig. 1.

Fig. 1. Double digestion of PlacI-ofp

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]