Difference between revisions of "Part:BBa K3871006:Design"

 
 
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===Design Notes===
 
===Design Notes===
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        This part is a synthetic variant of the rplD (ribosomal protein L4) promoter endogenous to <i>Bacillus subtilis</i> strain PY79. The promoter sequence was modified to
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    optimize the expression of the target gene under its control in <i>Bacillus subtilis</i> against <i>E. coli</i> using the
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    <a href="https://2021.igem.org/Team:TAU_Israel" style="padding-right: 0px;">Communique</a> tool developed
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    by the 2021 TAU Israel team. You may
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    read more about the optimization model
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    <a href="https://2021.igem.org/Team:TAU_Israel/Model" style="padding-right: 0px;">here.</a>
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<p>For more information on this part, please consult the 2021 TAU Israel wiki results page found <a href = "https://2021.igem.org/Team:TAU_Israel/Results" style="padding-right: 0px;">here.</a> Information about experimental procedures can be found <a href = "https://2021.igem.org/Team:TAU_Israel/Notebook" style="padding-right: 0px;">here</a> and <a href = "https://2021.igem.org/Team:TAU_Israel/Proof_Of_Concept" style="padding-right: 0px;">here.</a></p>
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Latest revision as of 00:48, 22 October 2021


modified Bacillus subtilis rplD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a synthetic variant of the rplD (ribosomal protein L4) promoter endogenous to Bacillus subtilis strain PY79. The promoter sequence was modified to optimize the expression of the target gene under its control in Bacillus subtilis against E. coli using the Communique tool developed by the 2021 TAU Israel team. You may read more about the optimization model here.


For more information on this part, please consult the 2021 TAU Israel wiki results page found here. Information about experimental procedures can be found here and here.



Source

The original promoter is endogenous to Bacillus subtilis strain PY79.

References