Difference between revisions of "Part:BBa K3795000:Design"
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===Source=== | ===Source=== | ||
| − | It was designed by the iGEM 2021 team Rio_UFRJ_Brazil through the combination of promising Dengue epitopes in order to minimize cross-reaction with anti-Zika antibodies. After the design, it was synthesized by Genscript. | + | It was designed by the iGEM 2021 team Rio_UFRJ_Brazil through the combination of promising Dengue epitopes in order to minimize cross-reaction with anti-Zika antibodies. After the design, it was synthesized by Genscript. The initial epitopes were collected from previous work from AnandaRao[1,2], which were submited to bioinformatics analysis in minimize cross-reaction. |
===References=== | ===References=== | ||
#AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N. A custom-designed recombinant multiepitope protein as a dengue diagnostic reagent. Protein Expression and Purification. 2005;41(1):136–47. | #AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N. A custom-designed recombinant multiepitope protein as a dengue diagnostic reagent. Protein Expression and Purification. 2005;41(1):136–47. | ||
#AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N. Recombinant multiepitope protein for early detection of dengue infections. Clinical and Vaccine Immunology. 2006;13(1):59–67. | #AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N. Recombinant multiepitope protein for early detection of dengue infections. Clinical and Vaccine Immunology. 2006;13(1):59–67. | ||
Latest revision as of 00:25, 22 October 2021
DME-C: Multi-epitope protein for anti Dengue antibodies detection
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 276
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is codon-optimized for expression in Escherichia coli. Besides, restriction sites for Biobrick Standard Assembly (EcoRI, PstI, XbaI, SpeI) and also BamHI and NocI were removed.
Source
It was designed by the iGEM 2021 team Rio_UFRJ_Brazil through the combination of promising Dengue epitopes in order to minimize cross-reaction with anti-Zika antibodies. After the design, it was synthesized by Genscript. The initial epitopes were collected from previous work from AnandaRao[1,2], which were submited to bioinformatics analysis in minimize cross-reaction.
References
- AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N. A custom-designed recombinant multiepitope protein as a dengue diagnostic reagent. Protein Expression and Purification. 2005;41(1):136–47.
- AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N. Recombinant multiepitope protein for early detection of dengue infections. Clinical and Vaccine Immunology. 2006;13(1):59–67.