Difference between revisions of "Part:BBa K245114:Experience"
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   Through the modeling built with substituting parameters from published articles, the growth of <i>E. coli</i> and <i>P. gingivalis</i> under the effect of LL-37 would be inhibited significantly. However, the figure also shows that <i>E. coli</i> could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model. |    Through the modeling built with substituting parameters from published articles, the growth of <i>E. coli</i> and <i>P. gingivalis</i> under the effect of LL-37 would be inhibited significantly. However, the figure also shows that <i>E. coli</i> could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model. | ||
− | [[File:T-- | + | [[File:T--NCTU Formosa--bacteria V.S. time666.png|500px|thumb|center|'''Figure 2. The growth curve of <i>E. coli</i> and <i>P. gingivalis</i> |
]] | ]] | ||
− | [[File:T--NCTU_Formosa--bacteria_with_DenTeeth.png|500px|thumb|center|'''Figure 3. The growth curve of <i>E. coli</i> and <i>P. gingivalis</i> | + | [[File:T--NCTU_Formosa--bacteria_with_DenTeeth 666.png|500px|thumb|center|'''Figure 3. The growth curve of <i>E. coli</i> and <i>P. gingivalis</i> with DenTeeth |
]] | ]] | ||
   Because <i>P. gingivalis</i> was in the RG2, so we could not do the LL-37 functional test by inhibiting the growth of <i>P. gingivalis</i>. After reading some related papers, we found that the killing rate of <i>E. coli</i> was similar to that of P. gingivalis [4]. Based on these data, we determined to make E. coli, DH5α with pET32A, as the bacteria killed by DenTeeth in the LL-37 functional test. |    Because <i>P. gingivalis</i> was in the RG2, so we could not do the LL-37 functional test by inhibiting the growth of <i>P. gingivalis</i>. After reading some related papers, we found that the killing rate of <i>E. coli</i> was similar to that of P. gingivalis [4]. Based on these data, we determined to make E. coli, DH5α with pET32A, as the bacteria killed by DenTeeth in the LL-37 functional test. | ||
[[File:T--NCTU_Formosa--bacteriatable.png|500px|thumb|center|'''Table 1. Parameters of the sterilization system of LL37 | [[File:T--NCTU_Formosa--bacteriatable.png|500px|thumb|center|'''Table 1. Parameters of the sterilization system of LL37 | ||
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= Feeding Frequency Validation = | = Feeding Frequency Validation = |
Latest revision as of 03:02, 22 October 2021
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