Difference between revisions of "Part:BBa K3871001"
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<p> | <p> | ||
mCherry is a red fluorescent protein used as a reporter derived from the | mCherry is a red fluorescent protein used as a reporter derived from the | ||
| − | fluorophore DsRed, which | + | fluorophore DsRed, which was originally isolated from |
<i>Discosoma</i> sea anemones. It is commonly used due to its colour and | <i>Discosoma</i> sea anemones. It is commonly used due to its colour and | ||
photostability compared to other monomeric fluorophores. | photostability compared to other monomeric fluorophores. | ||
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This part is a variant of mCherry, the sequence of which was modified to | This part is a variant of mCherry, the sequence of which was modified to | ||
optimize its expression in <i>Bacillus subtilis</i> against <i>E. coli</i> using the | optimize its expression in <i>Bacillus subtilis</i> against <i>E. coli</i> using the | ||
| − | <a href="https://2021.igem.org/Team:TAU_Israel" style="padding-right: 0px;">Communique</a>tool developed | + | <a href="https://2021.igem.org/Team:TAU_Israel" style="padding-right: 0px;">Communique</a> tool developed |
by the 2021 TAU Israel team. This specific variant was optimized based on | by the 2021 TAU Israel team. This specific variant was optimized based on | ||
codon usage bias in highly expressed genes in <i>B. subtilis</i>. You may | codon usage bias in highly expressed genes in <i>B. subtilis</i>. You may | ||
read more about the optimization model | read more about the optimization model | ||
| − | <a href="https://2021.igem.org/Team:TAU_Israel/Model" | + | <a href="https://2021.igem.org/Team:TAU_Israel/Model" style="padding-right: 0px;">here.</a> |
</p> | </p> | ||
| + | <br> | ||
| + | <p>For more information on this part, please consult the 2021 TAU Israel wiki results page found <a href = "https://2021.igem.org/Team:TAU_Israel/Results" style="padding-right: 0px;">here.</a> Information about experimental procedures can be found <a href = "https://2021.igem.org/Team:TAU_Israel/Notebook" style="padding-right: 0px;">here</a> and <a href = "https://2021.igem.org/Team:TAU_Israel/Proof_Of_Concept" style="padding-right: 0px;">here.</a></p> | ||
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<partinfo>BBa_K3871001 short</partinfo> | <partinfo>BBa_K3871001 short</partinfo> | ||
<partinfo>BBa_K3871001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3871001 SequenceAndFeatures</partinfo> | ||
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Latest revision as of 02:19, 22 October 2021
Description
mCherry is a red fluorescent protein used as a reporter derived from the fluorophore DsRed, which was originally isolated from Discosoma sea anemones. It is commonly used due to its colour and photostability compared to other monomeric fluorophores.
This part is a variant of mCherry, the sequence of which was modified to optimize its expression in Bacillus subtilis against E. coli using the Communique tool developed by the 2021 TAU Israel team. This specific variant was optimized based on codon usage bias in highly expressed genes in B. subtilis. You may read more about the optimization model here.
For more information on this part, please consult the 2021 TAU Israel wiki results page found here. Information about experimental procedures can be found here and here.
mCherry, CAI optimized for Bacillus subtilis
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]