Difference between revisions of "Part:BBa K175004:Design"

 
(→‎Design Notes)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K175004 short</partinfo>
 
<partinfo>BBa_K175004 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
None
 
−
 
  
 +
Original gene has a biobrick cut site at 169.
 +
Codon optimized for E.coli with upgene.
  
 
===Source===
 
===Source===

Latest revision as of 11:47, 8 July 2009

I-PpoI homing endonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 166


Design Notes

Original gene has a biobrick cut site at 169. Codon optimized for E.coli with upgene.

Source

From slime mold (Physarum polycephalum), http://www.ncbi.nlm.nih.gov/protein/102295?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum

References