Difference between revisions of "Part:BBa K3722002"
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===Instruction=== | ===Instruction=== | ||
Latest revision as of 23:22, 21 October 2021
MaFMO
Monooxygenase hydroxylates the C3 position of the indole ring and forms 6-Br-indoxyl.
Biological function
Monooxygenase from Methylophaga aminisulfidivorans (MaFMO). And flavin is used as a cofactor when MaFMO hydroxylates the C3 position of the indole ring and forms indigo. Indigo has a maximum absorption peak at 606nm.
Instruction
The coding sequence of target gene was inserted into an expression vectors with BBa_K3722000 to obtain BBa_K3722011. We transformed the constructed plasmid into E. coli BL21 (DE3) to verify its successful heterologous expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]