Difference between revisions of "Part:BBa K4006000:Design"

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===Source===
 
===Source===
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This backbone is from Wannathong et. al.
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This part is codon optimized from Team X's 20XX team's BBa_XXXXXXXX. The part is codon optimzed for Chlamydomonas reinhardtii.
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===References===
 
===References===
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Wannathong, T., Waterhouse, J.C., Young, R.E.B. et al. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii . Appl Microbiol Biotechnol 100, 5467–5477 (2016). https://doi.org/10.1007/s00253-016-7354-6

Latest revision as of 23:01, 21 October 2021


pASapI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2445
    Illegal XbaI site found at 3821
    Illegal PstI site found at 3833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4361
    Illegal NheI site found at 5790
    Illegal NheI site found at 6111
    Illegal PstI site found at 3833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3815
    Illegal XhoI site found at 3739
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2445
    Illegal XbaI site found at 3821
    Illegal PstI site found at 3833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2445
    Illegal XbaI site found at 3821
    Illegal PstI site found at 3833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1319
    Illegal BsaI site found at 3923
    Illegal BsaI.rc site found at 6517
    Illegal SapI.rc site found at 3800


Design Notes

This plasmid was first made type IIS compatible with part BBa_K4006002.

Source

This backbone is from Wannathong et. al.

References

Wannathong, T., Waterhouse, J.C., Young, R.E.B. et al. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii . Appl Microbiol Biotechnol 100, 5467–5477 (2016). https://doi.org/10.1007/s00253-016-7354-6