Difference between revisions of "Part:BBa K3725082"

Latest revision as of 04:18, 18 December 2021

Improved Fusarium Toehold Switch Sequence
The Improved Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the second lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the FRP1 gene of Fusarium oxysporum, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease F. oxysporum.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K3725082 short</partinfo>
+<br>
-The Improved Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the second lowest normalized ensemble defect rate possible. In the presence of the <i>F. Oxysporum</i> FRP1 gene, the hairpin loop will unfold and express the downstream GFP reporter protein.
+The Improved Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the second lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the FRP1 gene of <i>Fusarium oxysporum</i>, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease <i>F. oxysporum</i>.