Difference between revisions of "Part:BBa K3725080"

Latest revision as of 04:12, 18 December 2021

Fusarium Toehold Switch Sequence

The Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the FRP1 gene of Fusarium oxysporum, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease F. oxysporum.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3725080 short</partinfo>
-The Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. In the presence of the <i>F. Oxysporum</i> FRP1 gene, the hairpin loop will unfold and express the downstream GFP reporter protein.
+The Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the FRP1 gene of <i>Fusarium oxysporum</i>, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease <i>F. oxysporum</i>.
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