Difference between revisions of "Part:BBa K3747607:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The spacer was designed by choosing a Protospacer Adjacent Motif (PAM) sequence (5'-NGG-3'), within the non-template DNA strand sequence of the target gene. According to Batianis et al. [1], efficient gene repression could be achieved by choosing a PAM within the promoter sequence, which is why we designed the spacer to target the promoter sequence of the Cyo oxidase.
  
 
===Source===
 
===Source===
  
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<i>Pseudomonas putida</i> EM42
  
 
===References===
 
===References===
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[1] Batianis, C., Kozaeva, E., Damalas, S. G., Martín-Pascual, M., Volke, D. C., Nikel, P. I., & Martins Dos Santos, V. (2020). An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida. Microbial biotechnology, 13(2), 368–385. https://doi.org/10.1111/1751-7915.13533

Latest revision as of 23:21, 21 October 2021


Spacer targeting promoter Cyo oxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer was designed by choosing a Protospacer Adjacent Motif (PAM) sequence (5'-NGG-3'), within the non-template DNA strand sequence of the target gene. According to Batianis et al. [1], efficient gene repression could be achieved by choosing a PAM within the promoter sequence, which is why we designed the spacer to target the promoter sequence of the Cyo oxidase.

Source

Pseudomonas putida EM42

References

[1] Batianis, C., Kozaeva, E., Damalas, S. G., Martín-Pascual, M., Volke, D. C., Nikel, P. I., & Martins Dos Santos, V. (2020). An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida. Microbial biotechnology, 13(2), 368–385. https://doi.org/10.1111/1751-7915.13533