Difference between revisions of "Part:BBa K116401"
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Promoter of phoB in E.coli, an external phosphate regulated promoter that can be activated when encountering phosphate limitation.
 
Promoter of phoB in E.coli, an external phosphate regulated promoter that can be activated when encountering phosphate limitation.
  
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====Experience====
 
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Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017.
 
  
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[[File:Fjasknfkncjak.png|thumb|center|800px|Figure 1. Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM.]]
 
  
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'></span>
 
<partinfo>BBa_K116401 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K116401 SequenceAndFeatures</partinfo>
  

Latest revision as of 19:50, 17 December 2021


external phosphate sensing promoter

Promoter of phoB in E.coli, an external phosphate regulated promoter that can be activated when encountering phosphate limitation.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]