Difference between revisions of "Part:BBa K3971005"

Latest revision as of 21:51, 21 October 2021

spp - Sucrose-Phosphate Phosphatase gene from Synechocystis PCC 6803

Usage and Biology

spp codes for Sucrose-phosphate phosphatase (SPP), an enzyme involved in the sucrose biosynthesis pathway in the cyanobacteria Synechocystis PCC 6803[1].

Fructose-6-phosphate is converted into sucrose-6-phosphate by the action of sucrose-phosphate synthase (SPS). Sucrose-phosphate phosphatase (SPP) then catalyses the conversion of sucrose-6-phosphate into sucrose [2].

In Synechocystis PCC 6803, SPS and SPP are coded for by two separate genes, sps, and spp [1].

In S. elongatus, SPS and SPP are natively found as a fused protein, and this fusion protein is coded for by a single sps gene [3].

Metabolic pathway of sucrose biosynthesis in Synechococcus elongatus UTEX 2973. Figure adapted from: Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8.

References

[1] Du, Wei, et al. "Exploring the photosynthetic production capacity of sucrose by cyanobacteria." Metabolic engineering 19 (2013): 17-25. https://doi.org/10.1016/j.ymben.2013.05.001

[2] Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8.

[3] Duan, Y., Luo, Q., Liang, F. et al. Sucrose secreted by the engineered cyanobacterium and its fermentability. J. Ocean Univ. China 15, 890–896 (2016). https://doi.org/10.1007/s11802-016-3007-8

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-''spp'' codes for Sucrose-phosphate phosphatase (SPP),  an enzyme involved in the sucrose biosynthesis pathway in cyanobacterial species ''Synechocystis'' PCC 6803, ''Synechococcus elongatus'' PCC 7942, and ''Synechococcus elongatus'' UTEX 2973, among others.
+''spp'' codes for Sucrose-phosphate phosphatase (SPP),  an enzyme involved in the sucrose biosynthesis pathway in the cyanobacteria ''Synechocystis'' PCC 6803[1].
+Fructose-6-phosphate is converted into sucrose-6-phosphate by the action of sucrose-phosphate synthase (SPS). Sucrose-phosphate phosphatase (SPP) then catalyses the conversion of sucrose-6-phosphate into sucrose [2].
+In ''Synechocystis'' PCC 6803, SPS and SPP are coded for by two separate genes, ''sps'', and ''spp'' [1].
+In ''S. elongatus'',  SPS and SPP  are natively found as a fused protein, and this fusion protein is coded for by a single ''sps'' gene [3].
 [[File:T--IISER-Pune-India--sps-spp.jpg|thumb|300x370px|centre|Metabolic pathway of sucrose biosynthesis in Synechococcus elongatus UTEX 2973. Figure adapted from: Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8.]]
 ===References===
+[1] Du, Wei, et al. "Exploring the photosynthetic production capacity of sucrose by cyanobacteria." Metabolic engineering 19 (2013): 17-25. https://doi.org/10.1016/j.ymben.2013.05.001
+[2] Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8.
+[3] Duan, Y., Luo, Q., Liang, F. et al. Sucrose secreted by the engineered cyanobacterium and its fermentability. J. Ocean Univ. China 15, 890–896 (2016). https://doi.org/10.1007/s11802-016-3007-8
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