Difference between revisions of "Part:BBa K4055531"
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===Experiment=== | ===Experiment=== | ||
| − | + | [[File:T--Yucai_SZ--rs.jpeg|600px|thumb|center|The staining results of a dish of CsgA-Mfp3S-pep bacteria with IPTG (Left) and a blank control dish of amilCP bacteria (Right)]] | |
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After 9 days of SBF culture, THE MFP-CSGA was scanned by electron microscopy (compared with the sample on day 0). | After 9 days of SBF culture, THE MFP-CSGA was scanned by electron microscopy (compared with the sample on day 0). | ||
| − | + | [[File:T--Yucai_SZ--2.png|500px|thumb|center|SEM image showing the surface morphology of the biofilm (i.e., unmineralized). Scale bars:3μm]] | |
| − | + | [[File:T--Yucai_SZ--3.png|500px|thumb|center|SEM image showing the surface morphology of the mineralized composite (mineralization 7d). Scale bars: 3μm]] | |
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| − | + | [[File:T--Yucai_SZ--kuanghua.jpeg|600px|thumb|center|CsgA-Mfp3S-pep bacteria (Left) and amilCP bacteria (Right) cultured in SBF]] | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 01:03, 22 October 2021
pT7-B0034-csgA_linker_mfp3spep
Biofilm structural proteins in E. coli fused with mussel foot protein(Mfp) analogs bestowed the engineered biofilms with HA mineralization-promoting and interfacial binding roles. When a blue light-induced strain was used to grow functional biofilms with controlled spatial and biomass density, living mineralized materials with patterning and gradient features could be generated following a benign biomimetic HA mineralization process.
an Mfp3S protein could initiate HA mineralization and promote interfacial adhesion.CsgA–Mfp fusion proteins comprising a CsgA domain (major protein component of the E. coli biofilm31) and a C-terminal Mfp serial fusion domain were constructed.
Moreover, examination of in vivo mineralization by TEM confirmed that CsgA–Mfp3S-pep-expressing biofilms triggered denser mineral formation with more apparent crystalline features after 5 d of min-eralization . These results thus highlight the roles of the Mfp3S-pep fusion protein in promoting HA mineral formation and crystallization.
Experiment
After 9 days of SBF culture, THE MFP-CSGA was scanned by electron microscopy (compared with the sample on day 0).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]