Difference between revisions of "Part:BBa K3739001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | =====CBM===== | ||
+ | Cellulose enzymes have two domains, and the one that helps bind to cellulose is called cellulose binding module (CBM), and therefore it helps our fusion protein bind to cellulose-rich cell wall. Here we choose the CBM of CenA from ''Cellulomonas fimi'', which has been successfully expressed in ''Escherichia coli''. | ||
− | === | + | =====GFP===== |
− | + | Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 03:32, 22 October 2021
his-CBM-GFP
CBM can anchor onto cellulose and GFP can show whether CenA anchor or not becteria that is used to store our plasmid
Usage and Biology
CBM
Cellulose enzymes have two domains, and the one that helps bind to cellulose is called cellulose binding module (CBM), and therefore it helps our fusion protein bind to cellulose-rich cell wall. Here we choose the CBM of CenA from Cellulomonas fimi, which has been successfully expressed in Escherichia coli.
GFP
Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 286
Illegal AgeI site found at 376 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 439