Difference between revisions of "Part:BBa K3790150"
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<partinfo>BBa_K3790150 short</partinfo> | <partinfo>BBa_K3790150 short</partinfo> | ||
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===Introduction=== | ===Introduction=== | ||
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The fusion protein obtained by linking the C-terminus of Bst to the N-terminal of DbpA via a (G2S)3 linker. | The fusion protein obtained by linking the C-terminus of Bst to the N-terminal of DbpA via a (G2S)3 linker. | ||
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===Experimental Results=== | ===Experimental Results=== | ||
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The length of Bst-(G2S)3-DbpA DNA was 2013 bp, which is approximately 2020 bp after adding homology arms to both ends for PCR cloning. We isolated the target DNA by gel extraction for subsequent reactions. | The length of Bst-(G2S)3-DbpA DNA was 2013 bp, which is approximately 2020 bp after adding homology arms to both ends for PCR cloning. We isolated the target DNA by gel extraction for subsequent reactions. | ||
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<partinfo>BBa_K3790150 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3790150 SequenceAndFeatures</partinfo> | ||
Latest revision as of 17:23, 21 October 2021
Bst-(G2S)3-DbpA
Contents
Introduction
The fusion protein obtained by linking the C-terminus of Bst to the N-terminal of DbpA via a (G2S)3 linker.
Experimental Results
The length of Bst-(G2S)3-DbpA DNA was 2013 bp, which is approximately 2020 bp after adding homology arms to both ends for PCR cloning. We isolated the target DNA by gel extraction for subsequent reactions.
After ClonExpress® homologous recombination reaction, Fast-T1 competent bacteria were transformed with reactants, and spread onto LB plates with antibiotics. We picked colony after 16 h culturing plates at 37℃. We further grow clones in 2 ml LB liquid medium with antibiotics, 37℃ shaking overnight.
Mini-prep was performed the following day, and the resulting plasmids were sent to Sanger sequencing.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]