Difference between revisions of "Part:BBa K3802000:Design"

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This part is a composite of multiple parts from the registry. It was synthesized by a gene synthesizing company (IDT).
 
This part is a composite of multiple parts from the registry. It was synthesized by a gene synthesizing company (IDT).
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===References===
 
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Dziga, D., Wladyka, B., Zielińska, G., Meriluoto, J., & Wasylewski, M. (2012). Heterologous expression and characterisation of microcystinase. Toxicon, 59(5), 578–586. https://doi.org/doi.org/10.1016/j.toxicon.2012.01.001
 
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Maqsood, I., Shi, W., Wang, L., Wang, X., Han, B., Zhao, H., Nadeem, A. M., Moshin, B. S., Saima, K., Jamal, S. S., Din, M. F., Xu, Y., Tang, L., & Li, Y. (2018). Immunogenicity and Protective efficacy of orally administered recombinant Lactobacillus Plantarum expressing VP2 protein against IBDV in chicken. Journal of Applied Microbiology, 125(6), 1670–1681. https://doi.org/10.1111/jam.14073
 
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Narita, J., Okano, K., Tateno, T., Tanino, T., Sewaki, T., Sung, M.-H., Fukuda, H., & Kondo, A. (2005). Display of active enzymes on the cell surface of Escherichia coli using PgsA anchor protein and their application to bioconversion. Applied Microbiology and Biotechnology, 70(5), 564–572. https://doi.org/10.1007/s00253-005-0111-x
 
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Zhang, Y., Dong, W., Lv, Z., Liu, J., Zhang, W., Zhou, J., Xin, F., Ma, J., & Jiang, M. (2018). Surface display of bacterial Laccase CotA on Escherichia coli cells and its application in Industrial Dye Decolorization. Molecular Biotechnology, 60(9), 681–689. https://doi.org/10.1007/s12033-018-0103-6
 

Latest revision as of 17:04, 21 October 2021


PgsA-MlrA fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 827
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 813
    Illegal NgoMIV site found at 2323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 304
    Illegal BsaI.rc site found at 2160


Design Notes

PgsA is not orginally from E.coli, but rather from Bacillus subtilis. Therefore, we had to codon optimize the entire part to be compatible with E.coli.

Source

This part is a composite of multiple parts from the registry. It was synthesized by a gene synthesizing company (IDT).