Difference between revisions of "Part:BBa K3764002"

Latest revision as of 16:30, 21 October 2021

mRFP1 with T38stop (TAG) mutation

This part is a modified version of part E1010. The codon for Tyr at position 38 is changed to Amber stop codon (TAG).

It can be used as part of a sensor for Amber codon suppression experiments. Moreover, our team (iGEM Bulgaria 2021) used this part in combination with BBa_K3764003 to design a cloning-based system for monitoring the formation of unproductive chimeras and artifacts between highly similar DNA sequences in PCR amplifications. For more details about this system - see our wiki!

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 555
Illegal AgeI site found at 667
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3764002 short</partinfo>
-This part is s modified version of a part E1010. The codon for Tyr at position 38 is changed to Amber stop codon (TAG).
+This part is a modified version of part E1010. The codon for Tyr at position 38 is changed to Amber stop codon (TAG).
-It can be used as part of a sensor for Amber codon suppression experiments. Moreover, our team (iGEM Bulgaria 2021) used this part in combination with BBa_K3764002 to design a cloning-based system for monitoring the formation of unproductive chimeras and artifacts between highly similar DNA sequences in PCR amplifications.
+It can be used as part of a sensor for Amber codon suppression experiments. Moreover, our team (iGEM Bulgaria 2021) used this part in combination with BBa_K3764003 to design a cloning-based system for monitoring the formation of unproductive chimeras and artifacts between highly similar DNA sequences in PCR amplifications. For more details about this system - see our wiki!