Difference between revisions of "Part:BBa K4060505:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The construction of this part is according to igem assembly protocol.
+
We use PCR technique to clone the gene fragment from template pKA-207I10(Plasmid#79845) from Addgene. We add restriction enzyme cutting site that according to igem part assembly protocol RFC#10 to both sides of the gene fragment, then using these restriction enzyme cutting site to insert the gene fragment into vector pSB1C3.
  
  
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===Source===
 
===Source===
  
−
The source of the gene is from Addgene plasmid pKA-207I10.
+
The source of the gene is from Addgene plasmid pKA-207I10(Plasmid#79845).
  
 
===References===
 
===References===

Latest revision as of 02:31, 22 October 2021


BphP1 gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 416
    Illegal XhoI site found at 2166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 367
    Illegal NgoMIV site found at 402
    Illegal NgoMIV site found at 1650
    Illegal NgoMIV site found at 2152
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1207
    Illegal BsaI.rc site found at 2053


Design Notes

We use PCR technique to clone the gene fragment from template pKA-207I10(Plasmid#79845) from Addgene. We add restriction enzyme cutting site that according to igem part assembly protocol RFC#10 to both sides of the gene fragment, then using these restriction enzyme cutting site to insert the gene fragment into vector pSB1C3.


Source

The source of the gene is from Addgene plasmid pKA-207I10(Plasmid#79845).

References