Difference between revisions of "Part:BBa K3735888"
 
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<partinfo>BBa_K3735888 short</partinfo>
 
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In order to explore the optimal conditions for the production of SeNPs for BBa_K3735004, we used the orthogonal experimental model-Response surface model(more details see CO Model).
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<p>In order to explore the optimal conditions for the production of SeNPs for BBa_K3735004, we used the orthogonal experimental model-Response surface model <html><a href='https://2021.igem.org/Team:NAU-CHINA/Model/Conditions_Optimization_Model'>(more details see CO Model)</a></html>.</p>
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We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments.
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<p>We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments.</p>
  
 
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<p style="text-align: center!important;"><b>Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16°C,30°C,37°C and selenite concentration are 12mM,15mM,18mM. </b></p>
 
<p style="text-align: center!important;"><b>Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16°C,30°C,37°C and selenite concentration are 12mM,15mM,18mM. </b></p>
  
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Second according to preliminary results and setting gradients to explore the interaction between three different types of conditions—pH 6, 7, and 8, temperature 16。C, 30。C, and 37。C, selenite concentration 12, 15, and 18mM, and conducted experiments as shown in the Fig.2.</p>
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<p>Second, according to preliminary results and setting gradients to explore the interaction between three different types of conditions—pH 6, 7, and 8, temperature 16℃, 30℃, and 37℃, selenite concentration 12, 15, and 18mM, and conducted experiments as shown in the Fig.2.</p>
 
      
 
      
 
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<img src="https://2021.igem.org/wiki/images/0/04/T--NAU-CHINA--Project_Results.fig.6.png"width="700" height=""width="350" height=""/>
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<img src="https://2021.igem.org/wiki/images/d/d2/T--NAU-CHINA--ES.fig.8%EF%BC%88%E4%BF%AE%E6%AD%A3%EF%BC%89.png"width="700" height=""width="350" height=""/>
 
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<p style="text-align: center!important;"><b>Fig.2 Response surface experiment of SeNPs synthesis. </b></p>
 
<p style="text-align: center!important;"><b>Fig.2 Response surface experiment of SeNPs synthesis. </b></p>
  
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<p>We used response surface model to simulate the optimal SeNPs production conditions (details see <u>CO Model</u>（此处添加超链接至相关页面）). Finally, we obtained the optimal conditions and maximum yield as follows：
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<p>We used response surface model to simulate the optimal SeNPs production conditions <html><a href='https://2021.igem.org/Team:NAU-CHINA/Model/Conditions_Optimization_Model'>(more details see CO Model)</a>)</html>. Finally, we obtained the optimal conditions and maximum yield as follows：
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<p>Solutions</p>
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<br>Solutions:<br>
 
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<img src="https://static.igem.org/mediawiki/parts/e/e1/T--NAU-CHINA-biaoge2.png"width="700" height=""width="350" height=""/>
 
<img src="https://static.igem.org/mediawiki/parts/e/e1/T--NAU-CHINA-biaoge2.png"width="700" height=""width="350" height=""/>
 
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<p>By response surface model analysis, we simulated the optimal experimental conditions of SeNPs production: temperature at 30.27°C, selenite concentration at 14.33mM, pH at 6.75. Finally, the optimal SeNPs yield of 0.0305g was obtained. Fig. 3 is our final product .</p>
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By response surface model analysis, we simulated the optimal experimental conditions of SeNPs production: temperature at 30.27℃, selenite concentration at 14.33mM, pH at 6.75. Finally, the optimal SeNPs yield of 0.0315562g was obtained.</p>
  
 
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Latest revision as of 21:03, 21 October 2021


SeNPs production parts

In order to explore the optimal conditions for the production of SeNPs for BBa_K3735004, we used the orthogonal experimental model-Response surface model (more details see CO Model).

We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments.

Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16°C,30°C,37°C and selenite concentration are 12mM,15mM,18mM.

Second, according to preliminary results and setting gradients to explore the interaction between three different types of conditions—pH 6, 7, and 8, temperature 16℃, 30℃, and 37℃, selenite concentration 12, 15, and 18mM, and conducted experiments as shown in the Fig.2.

Fig.2 Response surface experiment of SeNPs synthesis.

We used response surface model to simulate the optimal SeNPs production conditions (more details see CO Model)). Finally, we obtained the optimal conditions and maximum yield as follows：
Solutions:

By response surface model analysis, we simulated the optimal experimental conditions of SeNPs production: temperature at 30.27℃, selenite concentration at 14.33mM, pH at 6.75. Finally, the optimal SeNPs yield of 0.0315562g was obtained.

Fig.3 the final product of our project.


Sequence and Features


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