Difference between revisions of "Part:BBa K3805209"

Latest revision as of 03:56, 22 October 2021

Bi-stable Switch

(Toggle Switch)

This part is a Bi-stable switch(toggle switch).It is composed of trc promoter(BBa_K3805208))[1], P2 promoter(BBa_I746104))[2], lacI- LVA (BBa_C0012))[3]and ompT-mcherry(BBa_K3805886))[4].

Usage and Biology

At the presence of autoinducing peptides(AIPs), P2 promoter will be activated by phosphorylated AgrA (BBa_K38055))[5]and initiate the expression of downstrem lacI-LVA and ompT. LacI-LVA inhibits trc promoter.As the concentration of AIP decreases, the downstream transcription of P2 promoter will be down-regulated and the inhibition of lacI-LVA on the trc promoter will thus be lifted.The trc promoter then initiates the its downstream expression.

Figure 1 Images under fluorescence microscope.(A)Before putting into AIPs, red fluorescence was observed.(B).After putting into AIP, red fluorescence disappeared,green fluorescence was observed. (fluorescence microscope 10x100)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1287
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3805209 short</partinfo>
+===(Toggle Switch)===
-This part is a toggle switch.It is composed of trc promoter(BBa_K3805208))[https://parts.igem.org/Part:BBa_K3805208], P2 promoter(BBa_I746104))[https://parts.igem.org/Part:BBa_I746104], lacI- LVA (BBa_C0012))[https://parts.igem.org/Part:BBa_C0012]and ompT-mcherry(BBa_K3805886))[https://parts.igem.org/Part:BBa_K3805886].
+This part is a Bi-stable switch(toggle switch).It is composed of trc promoter(BBa_K3805208))[https://parts.igem.org/Part:BBa_K3805208], P2 promoter(BBa_I746104))[https://parts.igem.org/Part:BBa_I746104], lacI- LVA (BBa_C0012))[https://parts.igem.org/Part:BBa_C0012]and ompT-mcherry(BBa_K3805886))[https://parts.igem.org/Part:BBa_K3805886].