Difference between revisions of "Part:BBa K239006:Design"
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<partinfo>BBa_K239006 short</partinfo> | <partinfo>BBa_K239006 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the | + | Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the promoter-distal FNR binding site. |
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | |||
+ | Bonnefoy V, DeMoss JA (1992). "Identification of functional cis-acting sequences involved in regulation of narK gene expression in Escherichia coli." Mol Microbiol 1992;6(23);3595-602. PMID: 1474901 | ||
+ | |||
+ | |||
+ | Engel, P., M. Trageser, and G. Unden. (1991) Reversible interconversion of | ||
+ | the functional state of the gene regulator FNR from Escherichia coli in vivo | ||
+ | by O2 and iron availability. Arch. Microbiol. 156:463–470. | ||
+ | |||
+ | |||
+ | Green, J., B. Bennett, P. Jordan, E. T. Ralph, A. J. Thomson, and J. R. | ||
+ | Guest. (1996) Reconstitution of the [4Fe-4S] cluster in FNR and demonstration | ||
+ | of the aerobic-anaerobic transcription switch in vitro. Biochem. J. 316: | ||
+ | 887–892. | ||
+ | |||
+ | |||
+ | Spiro, S., and J. R. Guest. (1987) Regulation and overexpression of the fnr | ||
+ | gene of Escherichia coli. J. Gen. Microbiol. 133:3279–3288. | ||
+ | |||
+ | |||
+ | Spiro, S., and J. R. Guest. (1990) FNR and its role in oxygen-regulated gene | ||
+ | expression in Escherichia coli. FEMS Microbiol. Rev. 75:399–428. | ||
+ | |||
+ | |||
+ | Kolesnikow T, Schröder I, Gunsalus RP. (1992) | ||
+ | Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum. | ||
+ | J Bacteriol. 1992 Nov;174(22):7104-11. |
Latest revision as of 18:27, 24 October 2009
Modified NarK promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the promoter-distal FNR binding site.
Source
Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.
References
Bonnefoy V, DeMoss JA (1992). "Identification of functional cis-acting sequences involved in regulation of narK gene expression in Escherichia coli." Mol Microbiol 1992;6(23);3595-602. PMID: 1474901
Engel, P., M. Trageser, and G. Unden. (1991) Reversible interconversion of
the functional state of the gene regulator FNR from Escherichia coli in vivo
by O2 and iron availability. Arch. Microbiol. 156:463–470.
Green, J., B. Bennett, P. Jordan, E. T. Ralph, A. J. Thomson, and J. R.
Guest. (1996) Reconstitution of the [4Fe-4S] cluster in FNR and demonstration
of the aerobic-anaerobic transcription switch in vitro. Biochem. J. 316:
887–892.
Spiro, S., and J. R. Guest. (1987) Regulation and overexpression of the fnr
gene of Escherichia coli. J. Gen. Microbiol. 133:3279–3288.
Spiro, S., and J. R. Guest. (1990) FNR and its role in oxygen-regulated gene
expression in Escherichia coli. FEMS Microbiol. Rev. 75:399–428.
Kolesnikow T, Schröder I, Gunsalus RP. (1992)
Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum.
J Bacteriol. 1992 Nov;174(22):7104-11.