Difference between revisions of "Part:BBa K3764000"
 
(2 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3764000 short</partinfo>
 
<partinfo>BBa_K3764000 short</partinfo>
  
−
This part contains a specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion. Once integrated into a BioBrick vector it will transform the plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE SELECTED VECTOR BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.
 
  
−
This part has no promoter! You should add one if you want to use the blue-white screening procedure with your newly generated T-vector.
+
This part contains a specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion. Moreover, this part also supports the blue-white screening procedure available for vectors like pUC19 and pBluescript. Once integrated into a BioBrick vector it will transform the corresponding plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE SELECTED VECTOR BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.
 +
 
 +
 
 +
This part has no promoter and RBS module! You should add one if you want to use the blue-white screening procedure with your newly generated T-vector.
 +
 
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:41, 21 October 2021


Module for generation of a T-vector


This part contains a specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion. Moreover, this part also supports the blue-white screening procedure available for vectors like pUC19 and pBluescript. Once integrated into a BioBrick vector it will transform the corresponding plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE SELECTED VECTOR BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.


This part has no promoter and RBS module! You should add one if you want to use the blue-white screening procedure with your newly generated T-vector.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 341
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]