Difference between revisions of "Part:BBa K3921008"

Latest revision as of 14:02, 21 October 2021

172-dicF-ccaR&ccaS

RNAi system containing PcpccG2-172 promoter.Under the green light of 520nm, CcaS (green circle) is self-phosphorylated, then the phosphate group (yellow circle) is transferred to the corresponding regulatory factor CcaR (red circle), and the phosphorylated CcaR binds to the cpcG2 promoter to start the expression of DicF and cell division is inhibited. when induced by red light of 660nm, DicF is not expressed and cells divide normally.

Figure 1. Schematics of the 172_dicF_ccaR&S circuit.

we used green light to induce for about 7.5 hours, and found that the MG1655 had formed a filamentous shape (Fig. 2B). Then, we induced with red light for 10 hours, at which time some of the cells in the picture had returned to normal division (Fig. 2C). However, the cells in the lower part of the image still appear filamentous, which may be due to the fact that the observed sample is in the center and there are other samples around to block the light. Subsequently, we observed the marginal samples (Fig. 3) and found that almost all the cells in the marginal samples had returned to normal division (Fig. 2D).

Figure 2. Real-time observation microscope (40×) map of 172_dicF_ccaR&S MG1655. (A) Initial cell morphology at 0 h. (B) Cell morphology induced by green light for about 7.5 h. (C) Cell morphology induced by conversion of red light for 12h. (A), (B) and (C) are all intermediate observation objects. (D) Morphology of marginal cells induced by conversion of red light for 12h.

Figure 3. Samples for observation.

Sequence and Features