Difference between revisions of "Part:BBa K3777029"
 
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<partinfo>BBa_K3777029 short</partinfo>
 
<partinfo>BBa_K3777029 short</partinfo>
  
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Basic biosensor device for tetracycline detection.
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A circuit that can improve the detection limit of erythromycin.
  
 
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<b><font size="3">Usage and Biology</font></b>
 
<b><font size="3">Usage and Biology</font></b>
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<br>The genetic circuit was composed of a coding sequence of  tetracycline repressor  which was inserted into an expression vectors with a consitive promoter(BBa_J23114) and RBS(BBa_K3777030), as well as 3WJdB(BBa_K3777000) under the control of T7 promoter (BBa_K3777006).  The terminator we used were BBa_B0010 and BBa_M36305.(Fig 1)
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<br>Compared to our another part BBa_K3777028,this part add a gene called mphA, which can improve the detection limit of erythromycin
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<br>When tet was absent, TetR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene.
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<br>According to the paper we reference,it is said that trapping erythromycin within Escherichia coli through phosphorylation increases the sensitivity of its biosensor (MphR) by approximately 10-fold.  
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<br>We expressed this circuit in the <i>E. coli </i> BL21(DE3) cells for tetracycline detection. Thus we could roughly deduce the concentration of the antibiotics in the sample according to the fluorescence intensity.  
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https://static.igem.org/mediawiki/parts/thumb/2/22/LuxR-Plux-dCas9-mphA.PNG/798px-LuxR-Plux-dCas9-mphA.PNG
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https://static.igem.org/mediawiki/parts/thumb/3/3d/Tetr-tetO-3WJdB.PNG/799px-Tetr-tetO-3WJdB.PNG  
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<br>                        
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<br>                           Fig.1  Schematic overview of the genetic circuit.
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<br>Referernce:Miller Corwin A,Ho Joanne M,Parks Sydney E,Bennett Matthew R. Macrolide Biosensor Optimization through Cellular Substrate Sequestration.[J]. ACS synthetic biology,2021,10(2)
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<br><b><font size="3">Results</font></b>
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<br>To verify the functionality of the biosensor, we performed a plate-reader experiment and measured optical density and fluorescence intensity every hour. We observed a correlation between concentration of antibiotics in the sample and intensity of fluorescent signal.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 18:05, 21 October 2021


luxR-Plux-dCas9-mphA

A circuit that can improve the detection limit of erythromycin.

Usage and Biology
Compared to our another part BBa_K3777028,this part add a gene called mphA, which can improve the detection limit of erythromycin
According to the paper we reference,it is said that trapping erythromycin within Escherichia coli through phosphorylation increases the sensitivity of its biosensor (MphR) by approximately 10-fold. 798px-LuxR-Plux-dCas9-mphA.PNG

Referernce:Miller Corwin A,Ho Joanne M,Parks Sydney E,Bennett Matthew R. Macrolide Biosensor Optimization through Cellular Substrate Sequestration.[J]. ACS synthetic biology,2021,10(2)
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2204
    Illegal NheI site found at 5364
    Illegal NheI site found at 5387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4483
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5951
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1022