Difference between revisions of "Part:BBa K3714016"
 
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<partinfo>BBa_K3714016 short</partinfo>
 
<partinfo>BBa_K3714016 short</partinfo>
  
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An oligonucleotide designed for inhibit aptazyme activity temporarily by toehold meditated strand displacing (TMSD) reaction. It will denature an aptazyme (BBa_K3714010) by hybridization.  
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An oligonucleotide designed for inhibit aptazyme activity temporarily by toehold meditated strand displacing (TMSD) reaction. It will denature an aptazyme (BBa_K3714010) by hybridization. <br/>
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 12:18, 21 October 2021


Stop oligo

An oligonucleotide designed for inhibit aptazyme activity temporarily by toehold meditated strand displacing (TMSD) reaction. It will denature an aptazyme (BBa_K3714010) by hybridization.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


K3714016.png
Figure.1 | Verification of stop oligo. The aptazyme was produced by a T7 in vitro transcription system. Gardiquimod is an inhibitor of the cleavage activity. The product was run on a denatured PAGE.