Difference between revisions of "Part:BBa K3712022"
 
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<center><img src="https://2021.igem.org/wiki/images/5/5f/T--HUST2-China--SDS-PAGE_of_Wild-type_TLR2-27ELP%2C_Optimized_TLR2-27_ELP_expression_results.png" style="width:646px;height:544px"></center>
 
<center><img src="https://2021.igem.org/wiki/images/5/5f/T--HUST2-China--SDS-PAGE_of_Wild-type_TLR2-27ELP%2C_Optimized_TLR2-27_ELP_expression_results.png" style="width:646px;height:544px"></center>
  
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<p><b>Figure 1.</b>SDS-PAGE of Wild-type TLR2-27ELP, Optimized TLR2-27 ELP expression results. Near the 25kDa marker, the binds of Wild-type TLR2-27ELP and Optimized TLR2-27 ELP after induction are darker than the expression system without induction.</p>
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<p><b>Figure 1.</b>SDS-PAGE of Wild-type TLR2-27ELP, Optimized TLR2-27 ELP expression results. Near the 25kDa marker, the binds of Wild-type TLR2-27 ELP and Optimized TLR2-27 ELP after induction are darker than the expression system without induction.</p>
  
 
<h1>Future direction</h1>
 
<h1>Future direction</h1>
  
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<p>Unfortunately, due to time issues, we have no way to complete the purification of TLR2 antagonist. In the future, we will try different linkers to connect Wild-type/Optimized TLR2 antagonist and 27ELP.</p>
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<p>Unfortunately, due to time issues, we have no way to complete the purification of TLR2 antagonist. In the future, we will try different linkers to connect Wild-type/Optimized TLR2 antagonist and 27 ELP.</p>
  
 
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Latest revision as of 19:52, 21 October 2021


Wild-type TLR2 antagonist-27 ELP

USAGE AND BIOLOGY

We connected the TLR2 antagonist to 27ELP in order to verify the ability of the TLR2 antagonist to bind to acylated lipoproteins and the heat-responsive aggregation effect of ELP after TLR2 and ELP were connected.

RESULTS

Part:BBa_K3712022

Figure 1.SDS-PAGE of Wild-type TLR2-27ELP, Optimized TLR2-27 ELP expression results. Near the 25kDa marker, the binds of Wild-type TLR2-27 ELP and Optimized TLR2-27 ELP after induction are darker than the expression system without induction.

Future direction

Unfortunately, due to time issues, we have no way to complete the purification of TLR2 antagonist. In the future, we will try different linkers to connect Wild-type/Optimized TLR2 antagonist and 27 ELP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178