Difference between revisions of "Part:BBa K3874003"
 
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<partinfo>BBa_K3874003 short</partinfo>
 
<partinfo>BBa_K3874003 short</partinfo>
  
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We add BBa_K243004(GGSG), a linker between our Sericin repeat. The protein will be folded well with the linker.
+
This part is to code a sericin like protein. The sequence of this protein has many repeat and this part is the 8 repeat unit.
 +
We add BBa_K243004(GGSG), a linker between each Sericin repeat. The protein will be folded well with the linker.
  
 
[[File:T--BJ101ID--3.png|400px|thumb|left|Figure 1. The Schematic cartoon of the DNA construct of BBa_K3874001.]]
 
[[File:T--BJ101ID--3.png|400px|thumb|left|Figure 1. The Schematic cartoon of the DNA construct of BBa_K3874001.]]

Latest revision as of 06:01, 21 October 2021


To add Linker between single repeat

This part is to code a sericin like protein. The sequence of this protein has many repeat and this part is the 8 repeat unit. We add BBa_K243004(GGSG), a linker between each Sericin repeat. The protein will be folded well with the linker.

Figure 1. The Schematic cartoon of the DNA construct of BBa_K3874001.
Figure 2. Western Blot Data prove IPTG 0.5mM in 18℃ and cultivating for 20h is the most proper condition and we use this condition in latter experiment..

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]