Difference between revisions of "Part:BBa K3739027"
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<partinfo>BBa_K3739027 short</partinfo>
 
<partinfo>BBa_K3739027 short</partinfo>
  
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We anchor LCI-KR2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use <partinfo>BBa_K3739057</partinfo> to construct the expression system and anchor LCI-KR2 on the surface of VnDX.
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We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use <partinfo>BBa_K3739057</partinfo> to construct the expression system and anchor LCIKR-2 on the surface of VnDX.
  
 
===Biology===
 
===Biology===
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LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCI-KR2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCI-KR2 is fused with LamB used as a sandwich-anchoring motif so that LCI-KR2 can be displayed on the surface of VnDX.
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LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX.
 
   
 
   
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===Characterization===
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===Usage===
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====1. Identification====
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Here, we use BBa_3739027 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.  
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After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.
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Latest revision as of 19:57, 21 October 2021


LamB1-LC1KR2-LamB2-his

We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use BBa_K3739057 to construct the expression system and anchor LCIKR-2 on the surface of VnDX.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX.

Usage

Here, we use BBa_3739027 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 760