Difference between revisions of "Part:BBa K3739056"
 
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LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. The linker is a meaningless short peptide that we use as a control. The linker is fused with LamB used as a sandwich-anchoring motif so that the linker can be displayed on the surface of VnDX.
 
LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. The linker is a meaningless short peptide that we use as a control. The linker is fused with LamB used as a sandwich-anchoring motif so that the linker can be displayed on the surface of VnDX.
 
   
 
   
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===Characterization===
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===Usage===
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====1. Identification====
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Here, we use BBa_3739020 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.  Due to the limited time, we did not get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design.
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After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.
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Latest revision as of 20:06, 21 October 2021


J23100-B0030-LamB1-linker-LamB2-his-B0010

This is another anchored proteins onto membranes through LamB and use his tag to comformation. We use BBa_K3739056 and his tag to verify LamB's function which can anchor proteins onto membranes.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. The linker is a meaningless short peptide that we use as a control. The linker is fused with LamB used as a sandwich-anchoring motif so that the linker can be displayed on the surface of VnDX.

Usage

Here, we use BBa_3739020 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene. Due to the limited time, we did not get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 824