Difference between revisions of "Part:BBa K3783000:Experience"
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| − | <p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli | + | <p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli</i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression. |
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Latest revision as of 02:39, 21 October 2021
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Applications of BBa_K3783000
OhioState Application
The promoter was cloned into a luciferase reporter and then transformed into a strain of E.coli expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression.
User Reviews
UNIQ0c49fb2c12c57a54-partinfo-00000001-QINU UNIQ0c49fb2c12c57a54-partinfo-00000002-QINU