Difference between revisions of "Part:BBa K3725210"
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Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part [https://parts.igem.org/Part:BBa_K3725220 BBa_K3725220]is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. This was designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing. | Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part [https://parts.igem.org/Part:BBa_K3725220 BBa_K3725220]is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. This was designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing. | ||
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<partinfo>BBa_K3725210 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3725210 SequenceAndFeatures</partinfo> | ||
This sequence is only the promoter sequence. For the composite part with sfGFP, visit part [https://parts.igem.org/Part:BBa_K3725220 BBa_K3725220]. | This sequence is only the promoter sequence. For the composite part with sfGFP, visit part [https://parts.igem.org/Part:BBa_K3725220 BBa_K3725220]. | ||
Latest revision as of 16:53, 20 October 2021
Phosphorylated NarL-Specific Promoter Sequence (pNar)
pNar is a regulatory promoter responsive to phosphorylated NarL. This gene is sourced from Bacillus Subtilis; however, it works with the DNA-binding response regulator NarL from the E. coli Nar Operon.
Usage and Biology
Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part BBa_K3725220is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. This was designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This sequence is only the promoter sequence. For the composite part with sfGFP, visit part BBa_K3725220.