Difference between revisions of "Part:BBa K3771040"

Latest revision as of 03:18, 22 October 2021

PpspA-CDO1

Description

This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CDO1

Biology

The ompA promoter facilitates the constitutive expression of OmpA/OprF. Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CDO1. CDO1 converts L-cysteine into L-cysteine sulfonic acid in the taurine synthesis L-cysteine sulfinic acid pathway [2].

Fig. 1. Taurine pathways in E. coli

Usage

We ligased the cs fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.

Reference

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.xhttps://pubmed.ncbi.nlm.nih.gov/16045608/

2. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093https://pubmed.ncbi.nlm.nih.gov/30516051/
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 12
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 591
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 <br><b style="font-size:1.3rem">Description</b><br>
 <br>This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CDO1<br><b style="font-size:1.3rem"><br>Biology</b><br>
-   <br>The <i>ompA</i> promoter facilitates the constitutive expression of OmpA/OprF. Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CDO1. CDO1 converts L-cysteine into L-cysteine sulfonic acid in the taurine synthesis L-cysteine sulfinic acid pathway [2].  <div style="width=100%; display:flex; align-items: center; justify-content: center;">
+   <br>The <i>ompA</i> promoter facilitates the constitutive expression of OmpA/OprF. Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the <i>pspA</i> promoter, initiating expression of CDO1. CDO1 converts L-cysteine into L-cysteine sulfonic acid in the taurine synthesis L-cysteine sulfinic acid pathway [2].  <div style="width=100%; display:flex; align-items: center; justify-content: center;">
 <img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:60%;">
 </div>
-<p align="center">Fig.1 Taurine pathways in <i>E. coli</i></p>
+<p align="center">Fig. 1. Taurine pathways in <i>E. coli</i></p>
    <br><b style="font-size:1.3rem">Usage</b><br>
    <br>We ligased the <i>cs</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.