Difference between revisions of "Part:BBa K3805999:Design"
 
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<partinfo>BBa_K3805999 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3805999 SequenceAndFeatures</partinfo>
  
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==Designed Notes==
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===Designed Notes===
  
 
To ensure the specificity of binding, 19bp sequence on the T7 promoter was selected as sgRNA. We design the special sgRNA on website http://crispr.dfci.harvard.edu/SSC/ which is owned by Harvard.
 
To ensure the specificity of binding, 19bp sequence on the T7 promoter was selected as sgRNA. We design the special sgRNA on website http://crispr.dfci.harvard.edu/SSC/ which is owned by Harvard.
  
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==Source==
+
===Source===
  
 
We expect to synthesize this fragment.
 
We expect to synthesize this fragment.

Latest revision as of 12:30, 20 October 2021

sgRNA bind to T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Designed Notes

To ensure the specificity of binding, 19bp sequence on the T7 promoter was selected as sgRNA. We design the special sgRNA on website http://crispr.dfci.harvard.edu/SSC/ which is owned by Harvard.

Source

We expect to synthesize this fragment.