Difference between revisions of "Part:BBa K3734011"

Latest revision as of 15:01, 21 October 2021

ELK

ELK is a kind of protein that can activate downstream gene expression after being regulated by phosphorylation.

ELK

ELK is generally dormant, when INSR accepts insulin to activate the MAPK pathway, Elk is phosphated into an active state, which can activate the expression of the gene downstream TRE.

1.Pattern diagram

Fig.1 The model diagram TetR-ELK

2.Experiments

2.1 Method

We used Western Blot to detect phosphorylation of ERK1/ERK2 in the phosphorylation pathway with ERK antibodies.

2.2 Results

Fig.2 ERK phosphorylation changes with different insulin treatment

Fig.3 ERK phosphorylation changes with different insulin treatment

3.Caution

Despite the length and the complication of phosphorylation pathway, the phosphorylation pathway of protein is a very short process, it is usually completed within minutes even seconds. The time of cracking the cells and collecting protein must be controlled precisely when detecting.

Reference：

[1]Haifeng Ye, Mingqi Xie, Shuai Xue.Self-adjusting synthetic gene circuit for correcting insulin resistance[J].Nat Biomed Eng.2017 Jan;1(1):0005.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 124
1000
COMPATIBLE WITH RFC[1000]

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                  <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 ERK phosphorylation changes with different insulin treatment</p>
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