Difference between revisions of "Part:BBa K4032005"
 
(6 intermediate revisions by 2 users not shown)
Line 4: Line 4:
  
 
This part contains the gene of the α-galactosidase-RFP fusion protein.
 
This part contains the gene of the α-galactosidase-RFP fusion protein.
−
 
  
 
===Usage and Biology===
 
===Usage and Biology===
  
−
This enzyme catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. https://www.uniprot.org/uniprot/P06720
+
This enzyme catalyzes the hydrolysis of terminal, non-reducing α-D-galactose residues in α-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. For more information, see [https://www.uniprot.org/uniprot/P06720 UniProt EC:3.2.1.22].
  
−
Red fluorescence of RFP is observed under the fluorescence microscope and natural light.
+
Red fluorescence of RFP is observed not only with a fluorescence microscope but also under the natural light source.
  
 +
The lac promoter, the RBS and the double terminator are added to this part, forming <partinfo>BBa_K4032101</partinfo>.
  
−
===Designs===
+
===Design===
  
 
This part expresses the α-galactosidase-RFP fusion protein.
 
This part expresses the α-galactosidase-RFP fusion protein.
  
−
α-galactosidase is from E.coli K-12 MG1655 and RFP is from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) .
+
α-galactosidase is from ''E. coli'' and RFP is from <partinfo>BBa_J04450</partinfo>.
  
−
We removed the stop codon of alpha-galactosidase and the start codon of RFP and connected the ends.
+
We removed the stop codon of the α-galactosidase gene and the start codon of the RFP gene and connected the two ends.
  
  

Latest revision as of 13:23, 21 October 2021


α-galactosidase-RFP

This part contains the gene of the α-galactosidase-RFP fusion protein.

Usage and Biology

This enzyme catalyzes the hydrolysis of terminal, non-reducing α-D-galactose residues in α-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. For more information, see UniProt EC:3.2.1.22.

Red fluorescence of RFP is observed not only with a fluorescence microscope but also under the natural light source.

The lac promoter, the RBS and the double terminator are added to this part, forming BBa_K4032101.

Design

This part expresses the α-galactosidase-RFP fusion protein.

α-galactosidase is from E. coli and RFP is from BBa_J04450.

We removed the stop codon of the α-galactosidase gene and the start codon of the RFP gene and connected the two ends.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1905
    Illegal AgeI site found at 2017
  • 1000
    COMPATIBLE WITH RFC[1000]