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| | GI is a kind of light-activated protein. If you shine blue light on it, it will change its own structure and will be able to combined with LOV. | | GI is a kind of light-activated protein. If you shine blue light on it, it will change its own structure and will be able to combined with LOV. |
| − | <!-- Add more about the biology of this part here
| + | |
| − | ===Usage and Biology===
| + | |
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| − | <meta charset="utf-8" />
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| − | <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no" />
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| − | <meta name="csu_china" content="csu_china" />
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| − | <!-- Google fonts-->
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| − | <link href="https://fonts.googleapis.com/css?family=Roboto+Slab:400,100,300,700" rel="stylesheet" type="text/css" />
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| − | <!-- Core theme CSS (includes Bootstrap)-->
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| − | <script src="http://code.jquery.com/jquery-latest.js"></script>
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| − | <script src="https://2021.igem.org/wiki/index.php?title=Template:CSU_CHINA/JS/script&action=raw&ctype=text/javascript"></script>
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| − | <script src="https://2021.igem.org/wiki/index.php?title=Template:CSU_CHINA/JS/scripts&action=raw&ctype=text/javascript"></script>
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| − | <style>
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| − | color: var(--yellow);
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| − | height: 2.5rem;
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| − | width: 100%;
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| − | height: 80vh;
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| − | #home>.pageHead>.pageHead-content>.pageHead-content__description {
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| − | margin: 6rem 10% 4rem 10%;
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| − | #navlist {
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| − | background-color: var(--dark);
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| − | padding: 2rem .5rem 4rem .5rem;
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| − | min-height: 55vh;
| + | |
| − | border-bottom-right-radius: 2rem;
| + | |
| − | position: relative;
| + | |
| − | text-align: center;
| + | |
| − | display: flex;
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| − | #navlist>.pageContent-navlist__head {
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| − | padding: 1rem 0 1rem 0;
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| − | margin: 1rem auto;
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| − | width: 3.5rem;
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| − |
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| − | #navlist>.pageContent-navlist__head>.title {
| + | |
| − | color: #ffffff;
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| − | font-size: 1.5rem;
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| − | font-weight: bold;
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| − | #navlist>.pageContent-navlist__item:hover,
| + | |
| − | #navlist>.active {
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| − | color: var(--dark);
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| − | padding: 2rem 5%;
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| − | font-size: 2rem;
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| − | line-height: 2.5rem;
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| − | color: var(--dark);
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| − | position: relative;
| + | |
| − | display: block;
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| − | width: 95%;
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| − | margin: 3rem auto 2rem auto;
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| − | line-height: 2.5rem;
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| − | position: relative;
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| − | display: block;
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| − | width: 95%;
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| − | #pagecontent .pageContent-main__title::after {
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| − |
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| − | #pagecontent .pageContent-main__textBox {
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| − | width: 90%;
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| − | font-size: 1.1rem;
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| − |
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| − | #pagecontent .pageContent-main__textBox .name {
| + | |
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| + | |
| − |
| + | |
| − | #pagecontent .pageContent-main__textBox .image {
| + | |
| − | width: 80%;
| + | |
| − | margin: 2rem 10%;
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| + | |
| − |
| + | |
| − | .pageContent-side .mytoc {
| + | |
| − | position: -webkit-sticky;
| + | |
| − | position: sticky;
| + | |
| − | top: 12rem;
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| − | background: transparent;
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| − | padding: 1.5rem;
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| − | border: none;
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| + | |
| − |
| + | |
| − | .pageContent-side .mytoc .toc_list {
| + | |
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| − | color: #888;
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| − |
| + | |
| − | .pageContent-side .mytoc .toc_list .toclist__item a {
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| − | .pageContent-side .mytoc .toc_list .toclist__item a:hover {
| + | |
| − | color: var(--primary)
| + | |
| − | }
| + | |
| − |
| + | |
| − | @media screen and (max-width: 800px) {
| + | |
| − | #home>.screen>#group1>.title {
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | #navbar>.logo {
| + | |
| − | padding: 0.5rem 0;
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| − | }
| + | |
| − | #collapse {
| + | |
| − | height: 0;
| + | |
| − | }
| + | |
| − | #home>.pageContent>.pageContent-side {
| + | |
| − | display: none;
| + | |
| − | }
| + | |
| − | #home>.screen>#group1 {
| + | |
| − | padding-top: 5vh;
| + | |
| − | }
| + | |
| − | #home>.pageContent>.pageContent-main>.profilelist>.profile {
| + | |
| − | width: calc(100% / 3)
| + | |
| − | }
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | <h2 class="pageContent-main__title">
| + | |
| | <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> | | <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> |
| | GI | | GI |
| Line 439: |
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| | </body> | | </body> |
| | </html> | | </html> |
| | + | |
| | + | <!-- Add more about the biology of this part here |
| | + | ===Usage and Biology=== |
| | | | |
| | <!-- --> | | <!-- --> |
| | <span class='h3bb'>Sequence and Features</span> | | <span class='h3bb'>Sequence and Features</span> |
| | <partinfo>BBa_K3734004 SequenceAndFeatures</partinfo> | | <partinfo>BBa_K3734004 SequenceAndFeatures</partinfo> |
| − |
| |
| | | | |
| | <!-- Uncomment this to enable Functional Parameter display | | <!-- Uncomment this to enable Functional Parameter display |
GI is a kind of light-activated protein. If you shine blue light on it, it will change its own structure and will be able to combined with LOV.
GI is a photosensitive protein, under the exposure of 450nm blue light, its structure will alter and can form a dimer with LOV. We used GI as a part of our blue light switch.
Fig.1 The model diagram of GIP-miR21T-GI-GAL4-4XmiR21TC
The duration of the combination of GI and LOV after the blue light exposure will alter as the illuminate method and the intensity of the blue light differ. We used strong light for a 30min exposure and then use weak light for another 30min
exposure.
[1]Masayuki Yazawa , Amir M Sadaghiani, Brian Hsueh.Induction of protein-protein interactions in live cells using light[J].Nat Biotechnol. 2009 Oct;27(10):941-5.
