Difference between revisions of "Part:BBa K4032006"
 
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__NOTOC__
 
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<partinfo>BBa_K4032006 short</partinfo>
 
<partinfo>BBa_K4032006 short</partinfo>
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NC_000913.3 malS alpha-amylase [Escherichia coli str. K-12 substr. MG1655] - Gene - NCBI (nih.gov)
 
  
 
Contents :
 
Contents :
  
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・The gene codes for the amylase-RFP fusion protein.
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・The gene cording for the amylase-RFP fusion protein (<partinfo>BBa_K4032003</partinfo>).
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・The native RBS(Part:BBa K523001 - parts.igem.org)
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・The native RBS from <partinfo>BBa_K523001</partinfo>
  
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For more information on the amylase gene ''malS'', see [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]
  
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Usage and Biology
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===Usage and Biology===
  
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This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).
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This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units. For more information, See [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3].
  
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In the case of this part, Red red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.
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In the case of this part, Red red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.
  
  
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The lac promoter and double terminator are added to this part forming BBa_K4032xx .
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The lac promoter and double terminator are added to this part, forming <partinfo>BBa_K4032104</partinfo> .
  
  

Latest revision as of 13:27, 21 October 2021


RBS+amylase-RFP

Contents :

・The gene cording for the amylase-RFP fusion protein (BBa_K4032003).

・The native RBS from BBa_K523001

For more information on the amylase gene malS, see NCBI: NC_000913.3

Usage and Biology

This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units. For more information, See NCBI: NC_000913.3.

In the case of this part, Red red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.


The lac promoter and double terminator are added to this part, forming BBa_K4032104 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1534
    Illegal AgeI site found at 2613
    Illegal AgeI site found at 2725
  • 1000
    COMPATIBLE WITH RFC[1000]