Difference between revisions of "Part:BBa K4032006"

Latest revision as of 13:27, 21 October 2021

RBS+amylase-RFP

Contents :

・The gene cording for the amylase-RFP fusion protein (BBa_K4032003).

・The native RBS from BBa_K523001

For more information on the amylase gene malS, see NCBI: NC_000913.3

Usage and Biology

This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units. For more information, See NCBI: NC_000913.3.

In the case of this part, Red red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.

The lac promoter and double terminator are added to this part, forming BBa_K4032104 .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1233
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 369
Illegal AgeI site found at 1534
Illegal AgeI site found at 2613
Illegal AgeI site found at 2725
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4032006 short</partinfo>
-NC_000913.3 malS alpha-amylase [Escherichia coli str. K-12 substr. MG1655] - Gene - NCBI (nih.gov)
 Contents :
-・The gene codes for the amylase-RFP fusion protein.
+・The gene cording for the amylase-RFP fusion protein (<partinfo>BBa_K4032003</partinfo>).
-・The native RBS(Part:BBa K523001 - parts.igem.org)
+・The native RBS from <partinfo>BBa_K523001</partinfo>
+For more information on the amylase gene ''malS'', see [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]
-Usage and Biology
+===Usage and Biology===
-       This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).
+This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units. For more information, See [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3].
-       In the case of this part, Red red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.
+In the case of this part, Red red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.
-The lac promoter and double terminator are added to this part forming BBa_K4032xx .
+The lac promoter and double terminator are added to this part, forming <partinfo>BBa_K4032104</partinfo> .