Difference between revisions of "Part:BBa K4032001"
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K4032001 short</partinfo>
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<partinfo>BBa_K4032003 short</partinfo>
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NC_000913.3 malS alpha-amylase [Escherichia coli str. K-12 substr. MG1655] - Gene - NCBI (nih.gov)
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NC_000913.3 https://www.ncbi.nlm.nih.gov/gene/948088
  
 
This part contains the gene of the amylase-RFP fusion protein.
 
This part contains the gene of the amylase-RFP fusion protein.
  
  
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Usage and Biology
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===Usage and Biology===
  
 
This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).
 
This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).
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This part expresses the fuse protein of amylase (from BBa_K523006 (https://parts.igem.org/Part:BBa_K523006) ) and RFP (from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) ). We removed the stop codon of amylase and the start codon of RFP and connected the ends.
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This part expresses the fuse protein of amylase (from <partinfo>BBa_K523006</partinfo>) and RFP (from <partinfo>BBa_J04450</partinfo>). We removed the stop codon of amylase and the start codon of RFP and connected the ends.
  
  
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The RBS is added to this part forming BBa_K4032xx.
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The RBS is added to this part forming <partinfo>BBa_K4032xx</partinfo>.
  
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The lac promoter, the RBS and the double terminator are added to this part forming BBa_K4032xx.
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The lac promoter, the RBS and the double terminator are added to this part forming <partinfo>BBa_K4032xx</partinfo>.
  
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4032001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4032003 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4032001 parameters</partinfo>
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<partinfo>BBa_K4032003 parameters</partinfo>
 
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Latest revision as of 09:39, 20 October 2021


amylase-RFP

NC_000913.3 https://www.ncbi.nlm.nih.gov/gene/948088

This part contains the gene of the amylase-RFP fusion protein.


Usage and Biology

This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).

In the case of this part, red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.


This part expresses the fuse protein of amylase (from BBa_K523006) and RFP (from BBa_J04450). We removed the stop codon of amylase and the start codon of RFP and connected the ends.


The RBS is added to this part forming No part name specified with partinfo tag..

The lac promoter, the RBS and the double terminator are added to this part forming No part name specified with partinfo tag..


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 336
    Illegal AgeI site found at 1501
    Illegal AgeI site found at 2580
    Illegal AgeI site found at 2692
  • 1000
    COMPATIBLE WITH RFC[1000]