Difference between revisions of "Part:BBa K4083011"

Latest revision as of 08:25, 21 October 2021

lacIq reversed

This is a mutated promoter of lacI, and it contributes to a higher level of transcription of lacI repressor [1]. For our RemiDuET project, we had reversed the lacIq promoter in the pRGPDuo2 plasmid [2].

Reference

[1] Cammack, R. (Ed.) (2006). Oxford Dictionary of Biochemistry and Molecular Biology. (2 ed.) Oxford University Press.

[2] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4083011 short</partinfo>
-dadas
+This is a mutated promoter of lacI, and it contributes to a higher level of transcription of lacI repressor [1]. For our RemiDuET project, we had reversed the lacIq promoter in the pRGPDuo2 plasmid [2].
+===Reference===
+[1] Cammack, R. (Ed.) (2006). Oxford Dictionary of Biochemistry and Molecular Biology. (2 ed.) Oxford University Press.
+[2] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514
 <!-- Add more about the biology of this part here