Difference between revisions of "Part:BBa K3712008"

 
 
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<partinfo>BBa_K3712008 short</partinfo>
 
<partinfo>BBa_K3712008 short</partinfo>
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===USAGE AND BIOLOGY===
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<p>Verify the thermal aggregation temperature of 27 ELP and the ability of TLR2 antagonist to bind to acylated lipoproteins with the connection of 27ELP and TLR2 antagonist.</p>
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===RESULTS===
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<p>Unfortunately, we have not been able to purify Wild-type TLR2-27 ELP and Optimized TLR2-27 ELP. The results of SDS-PAGE only show the molecular weight of 27 ELP. Therefore, we speculated that passing through the nickel column caused a break between Wild-type/Optimized TLR2 antagonist and 27 ELP.</p>
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===FUTURE DIRECTIONS===
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<p>After we purify the Wild-type/optimized TLR2 antagonist-27 ELP, we will verify that this product has a similar transition temperature with Wild-type BLP-7-27 ELP within ±0.5~1℃.</p>
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Verify the thermal aggregation temperature of 27ELP and the ability of TLR2 antagonist to bind to acylated lipoproteins with the connection of 27ELP and TLR2 antagonist.
 
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 13:14, 21 October 2021


Optimized TLR2 antagonist-27 ELP

USAGE AND BIOLOGY

Verify the thermal aggregation temperature of 27 ELP and the ability of TLR2 antagonist to bind to acylated lipoproteins with the connection of 27ELP and TLR2 antagonist.

RESULTS

Unfortunately, we have not been able to purify Wild-type TLR2-27 ELP and Optimized TLR2-27 ELP. The results of SDS-PAGE only show the molecular weight of 27 ELP. Therefore, we speculated that passing through the nickel column caused a break between Wild-type/Optimized TLR2 antagonist and 27 ELP.

FUTURE DIRECTIONS

After we purify the Wild-type/optimized TLR2 antagonist-27 ELP, we will verify that this product has a similar transition temperature with Wild-type BLP-7-27 ELP within ±0.5~1℃.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 172