Difference between revisions of "Part:BBa K3726070"

 
 
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<partinfo>BBa_K3726070 short</partinfo>
 
<partinfo>BBa_K3726070 short</partinfo>
  
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This is a composite part that corresponds with a level 1 MoClo part in accordance with  the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726065” L0_AcrbV2  which codifies a membrane transporter subunit , which has been optimized for the specific secretion of n-butanol. Within our project we have used AcrbV2 to enhance n-butanol secretion through cell membrane in order to cut down on toxicity generated by itself.
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The part is flanked by two connectors that will allow  the assembly of a Lv2 construct. In its upstream region the connector is 5'Con2 Bba_K2560066, and in its downstream region 3'Con2 Bba_K2560071 .
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Expression is controlled by the Medium-Strong inducible promoter pBAD “BBa_K3726076” which is regulated by the presence of Arabinose, and the strong synthetic RBS, RBS_PtaBs  “BBa_K3726092”
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:07, 20 October 2021


Lv.1 AcrbV2 - A

This is a composite part that corresponds with a level 1 MoClo part in accordance with the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726065” L0_AcrbV2 which codifies a membrane transporter subunit , which has been optimized for the specific secretion of n-butanol. Within our project we have used AcrbV2 to enhance n-butanol secretion through cell membrane in order to cut down on toxicity generated by itself.

The part is flanked by two connectors that will allow the assembly of a Lv2 construct. In its upstream region the connector is 5'Con2 Bba_K2560066, and in its downstream region 3'Con2 Bba_K2560071 .

Expression is controlled by the Medium-Strong inducible promoter pBAD “BBa_K3726076” which is regulated by the presence of Arabinose, and the strong synthetic RBS, RBS_PtaBs “BBa_K3726092”

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2033
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1634
    Illegal NheI site found at 1762
    Illegal PstI site found at 2033
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1573
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2033
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2033
    Illegal NgoMIV site found at 3169
    Illegal NgoMIV site found at 4685
    Illegal AgeI site found at 109
    Illegal AgeI site found at 1408
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1390