Difference between revisions of "Part:BBa K3791007"

Latest revision as of 21:06, 21 October 2021

gRNA Erythromycin

Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein (BBa_K2927006), and a spacer sequence which is specific for the desired gene to target (BBa_K3791002).

Usage, Biology and Characterization

As this part is a simplification of our final construct, usage, biology and characterization is explained there: BBa_K3791012

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 01:04, 20 October 2021 (view source) Aubafp (Talk \| contribs) (→‎Usage, Biology and Characterization) ← Older edit		Latest revision as of 21:06, 21 October 2021 (view source) Laurasanchezruiz (Talk \| contribs)
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	<partinfo>BBa_K3791007 short</partinfo>		<partinfo>BBa_K3791007 short</partinfo>

−	Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein, (<html><a href="https://parts.igem.org/Part:BBa_K2927006" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K2927006</a></html>) and a spacer sequence which is specific for the desired gene to target (<html><a href="https://parts.igem.org/Part:BBa_K3791002" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791002</a></html>).	+	Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein (<html><a href="https://parts.igem.org/Part:BBa_K2927006" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K2927006</a></html>), and a spacer sequence which is specific for the desired gene to target (<html><a href="https://parts.igem.org/Part:BBa_K3791002" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791002</a></html>).